-b-ecologies:
-bio
art lab
- sotware . hardware . wetware
BBrian Degger & Sneha Solanki
-January 30 2010--
-Polytechnic
lab
Brian
Degger and Sneha Solanki both follow the 'hands-on' approach and work
with biological materials, tools and artifacts which discuss life. Sneha
solanki presented from two points in her research; on hypothetical synthesis
and culturing your own 'Test Tube food' from plant tissue including.
Sneha also gave an introduction and demonstration on the wet lab processes
involved in plant tissue culturing and cloning. Brian
Degger presented his protoype DNA photocopying machine, a thermal cycler
which amplifies segments and strings of DNA. To extract DNA for his
photocopying machine Brian proposed a variety of methods; firstly as
an introductory guide to setting-up a DIYBio Lab, using FLOSS, educational
kits and auction sites and finally as a wet lab process brian by extracting
kiwi fruit DNA.
DOCUMENTATON
& RESOURCES **-
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Resources
# References
# Glossary
#
Keywords
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Demo
on DNA extraction using kiwi fruit. |
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Demo
on plant tissue culturing. |
Resources
DNA
Purification recipe
Materials-
1/4 of a shot glass full of your saliva
several drops of dish soap (look for sodium laurel sulfate in the
ingredients)
a pinch of table salt (1/16 of a teaspoon)
some contact-lens cleaning solution
meat tenderizer, or pineapple juice (optional)
Ice-cold 120-proof+ liquor (60%)(overproof rum works well)
Brian
Degger's DIY and DNA and ART talk references
pdf >>>
Sneha
solanki's Plant tissue culturing
recipe pdf >>>
References
DIYbio.
'Citizen scientists, amateur
biologists' - http://diybio.org
Open wetware
- http://openwetware.org
Synthetic
biology - http://syntheticbiology.org
SymbioticA
- http://www.symbiotica.uwa.edu.au/
Adam
Zaretsky - http://www.emutagen.com/
Natalie
Jerimejenko & Eugene Thackers -BIOTECH
hobbyist manual
Heath Bunting's 'Superweed'- http://www.irational.org/cta/superweed/
Critical Art Ensemble- http://www.critical-art.net/Biotech.html
Plants from Test Tubes: Introduction to Micropropagation- by Lydiane
Kyte and John Kleyn
Glossary
FLOSS. Free {Libre} Open Source software
Wet Lab- A laboratory where biological experimentation occurs,
termed wet becaus most of biological experimentation is with liquids
and is moist. Types follow:
- Bug Lab. Where bacteria are cultured, engineered and bacterial
DNA purified. Generally has a slight negative pressure, so that they
are contained and not released.
- Protein lab. Where proteins are purified, for example Growth
Hormone or Insulin.
Tissue culturing (plant or animal). A lab dedicated to culturing
the plant or animal cells where cross contamination with bacteria and
fungi is minimised.
DIYbio. A movement- Garage Biotechnology.
Synthetic
biology - Making,
designing and constructing
biological material / matter..
Keywords
culturing
. dead matter . ancient seeds . speculative . ownership . DNA . DIY
. art . ethics . transparency .
materiality . end user license . golden rice . future food . fish .
meat . plants . other ...
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